?Chitozen is a chitosan-coated coverslip immobilizing bacteria for microscopy without inducing bacteriostatic effects. It is compatible with 6-channel sticky slides for flow experiments. It is very helpful if you want to: 

• Image bacteria both still and alive under the microscope
• Maintain bacteria in a same focal plane for imaging while preserving their physiology 
• Renew culture medium, change growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the bacteria new comportment under the microscope
• Capture the moment: fix your bacteria and label them in situ to look at specific protein expression patterns during dynamic processes (i.e. motility, cell-cell interactions, predation, etc) 

Kit contents of Chitozen

Chitozen coverslips are compatible with 6-channel sticky slides to work in a closed system in static or dynamic flow conditions. Order you coverslips alone if you need to work in an open system (i.e. for AFM imaging) or to place them on depression slides. 


 

Applications of Chitozen
 

• Assay compatibility: 
  > Live imaging
  > Co-cultures (bacterial predators, immune cells)
  > Addition of external factors (e.g. antibiotics, chemicals, inhibitors)
  > Static or dynamic conditions
  > Immunostaining procedures
• Imaging modes: 
  > phase-contrast, 
  > epifluorescence, 
  > confocal, 
  > super-resolution microscopy, 
  > atomic force microscopy (AFM) - documentation & example pictures available on request
• Experimental outputs:
  > Behavioural changes: growth, elongation, cell division, fitness, colony/biofilm formation, predation, motility, etc
  > Single molecule imaging 
• Chitozen is efficient with the following bacteria: 
  > E. coli
  > Bacillus subtilis
  >  Caulobacter crescentus
  >  Corynebacterium glutamicum
  >  Helicobacter pylori 
  >  Mycobacterium smegmatis
  >  Myxococcus xanthus
  >  Pseudomonas aeruginosa
  >  Pseudomonas fluorescens
  >  Salmonella
  >  Staphylococcus aureus
  >  Vibrio cholerae
  This list is updated regularly according to feedback provided by researchers who use Chitozen.

Technical specifications of Chitozen

Material: glass
Format: 75.0 x 25.0 mm
Thickness: 0.170 mm / 1.5H
Lifetime: up to 12 months at room temperature, shielded from direct sunlight
Sticky slide dimensions (mm):


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